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Literature summary extracted from

  • Schopfer, C.R.; Kochs, G.; Lottspeich, F.; Ebel, J.
    Molecular characterization and functional expression of dihydroxypterocarpan 6a-hydroxylase, an enzyme specific for pterocarpanoid phytoalexin biosynthesis in soybean (Glycine max L.) (1998), FEBS Lett., 432, 182-186.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.93 CYP93A1 cDNA isolated from elicitor-induced soybean cells encodes the enzyme, CYP93A1 expressed in Saccharomyces cerevisiae Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.93 0.0001
-
 (6aR,11aR)-3,9-dihydroxypterocarpan recombinant enzyme expressed in yeast Glycine max
1.14.14.93 0.00016
-
 (6aR,11aR)-3,9-dihydroxypterocarpan enzyme from elicitor-treated soybean cells Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.93 microsome
-
 Glycine max
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.93 (6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2 Glycine max the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean (6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.93 Glycine max
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.93 cell culture
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.93 (6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
-
 Glycine max (6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.93 (6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2 the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean Glycine max (6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.93 30
-
 recombinant enzyme and soybean microsomes Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.93 7.4
-
 recombinant enzyme and soybean microsomes Glycine max